Can Aspergillus fumigatus conidia cause false-positive results in the galactomannan enzyme immunoassay test?

AUTOR(ES)

Cabana, Ângela Leitzke, Mendes, Josiara Furtado, Klafke, Gabriel Baracy, Brandolt, Tchana Martinez, Melo, Aryse Martins, Meireles, Mário Carlos Araújo, Xavier, Melissa Orzechowski

FONTE

Rev. Soc. Bras. Med. Trop.

DATA DE PUBLICAÇÃO

2018-06

RESUMO

Abstract INTRODUCTION: Several factors can cause false-positive results in the galactomannan (GM) test; however, others remain unknown. Presently, the impact of airborne contamination by Aspergillus conidia during enzyme-linked immunosorbent assay (ELISA) remains uninvestigated. METHODS: We studied 12 A. fumigatus isolates. Fungal conidia were serially diluted and tested for GM detection using the Platelia® Aspergillus enzyme immunoassay (EIA). RESULTS: The conidia concentration required for an EIA-positive result was 4.8 × 103 (median). CONCLUSIONS: This is the first study to evaluate the impact of environmental contamination on the Platelia® Aspergillus EIA assay. Only massive contamination can interfere with GM optical readings, suggesting that environmental contamination does not cause false-positive test results.

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