Byssochlamyopeptidase A, a Rennin-like Enzyme Produced by Byssochlamys fulva

AUTOR(ES)

Chu, Fun Sun

RESUMO

The production of a rennin-like enzyme by Byssochlamys fulva varied considerably with the isolates tested. Among the seven isolates tested, NRRL 2260, IMI 83277, and N.Y. 1 were good enzyme producers. The enzyme produced by isolate IMI 83277 was purified approximately 20-fold after (NH4)2SO4 precipitation, diethylaminoethyl-cellulose chromatography and Sephadex G-100 gel filtration. The partially purified enzyme has a pH optimum at 2.9 and a temperature optimum around 60 C. The enzyme appeared to be relatively stable at 40 C between pH 3.0 and pH 6.85. A name, byssochlamyopeptidase A, was proposed for this new enzyme. The milk-clotting activity of byssochlamyo-peptidase A is dependent on pH and appeared to be minimal at pH 6.2 or above. No extensive proteolysis has been observed during the milk-clotting process. The non-trichloroacetic acid-precipitable nitrogen titration curve on skim milk was comparable to that catalyzed by animal rennet.

Documentos Relacionados

• Byssotoxin A, a secondary metabolite of Byssochlamys fulva.
• Nonlogarithmic death rate calculations for Byssochlamys fulva and other microorganisms.
• Sialidase-like Enzymes Produced by Group A, B, C, G, and L Streptococci and by Streptococcus sanguis
• Modeling the Growth of Byssochlamys fulva on Solidified Apple Juice at Different Temperatures
• Rennin Enzyme of Endothia parasitica