Biochemical, molecular and behavioral alterations induced by neonatal hypoxia in mice. / Alterações bioquímicas, moleculares e comportamentais induzidas por hipóxia neonatal em camundongos

AUTOR(ES)
DATA DE PUBLICAÇÃO

2008

RESUMO

Introduction: Neonatal hypoxia-ischemia is one of the most significant factors that can lead to death in newborns. It has been linked with pathology in adult life, such as schizophrenia. Objectives: The aim of this work was to investigate long-term effects of neonatal hypoxia on body weight, behavior, antioxidant defense status, factors related to homocysteine metabolism in adult male mice. Methods: Seven day old male Swiss mice were submitted to: hypoxia (H), maternal separation (MS) or no handling (NH). When they completed 3 months old, behavioral evaluation were performed (activity boxes, plus-maze discriminative avoidance task, sleep recording). Blood catalase, superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione, vitamin B6, homocysteine and cysteine were measured as well as hippocampal and striatal catalase activity, and hipoccampal GPx and catalase expression. Data were analyzed by one-way ANOVA test or repeated measures ANOVA, followed by Duncan or Bonferroni adjusted t tests. Results: Adult mice exposed to neonatal hypoxia exhibited lower locomotor activity on the first minutes of evaluation and also a decrease in paradoxical sleep episodes duration during dark phase compared to NH group. Moreover an increase in time spent in the open arms of the maze was observed in both MS and H groups when compared to NH. With respect to biochemical parameters, only erythrocyte GPx activity was significantly reduced in the H group, whereas total glutathione was significantly lower in the MS group, both compared to NH group. There were no differences in hippocampal GPx and CAT expression. Groups MS and H presented higher weight gain than NH. Conclusion: Neonatal hypoxia produced long-lasting alterations in paradoxical sleep, body weight, neotic behavior and glutathione peroxidase activity whereas maternal 104 104 separation induced an enhanced anxiety-like behavior, reduction in total and body weight increase.

ASSUNTO(S)

psicobiologia estresse oxidativo neofobia glutationa peroxidase. hipóxia

ACESSO AO ARTIGO

Documentos Relacionados