Avaliação do efeito radioprotetor da vitamina "E" em glandulas parotidas de ratos : uma analise morfometrica / Evaluation of radioprotective effect of vitamin E in parotid gland of rats : morphometric analysis

AUTOR(ES)
DATA DE PUBLICAÇÃO

2009

RESUMO

The aim of this study was to evaluate, by morphometric analysis, the radioprotective effect of vitamin E in parotid glands of rats. For this purpose, ninety male rats (Rattus norvegicus, albinus, Wistar) were used, which were randomly divided into five experimental groups: Oil, corresponding to the animals that received olive oil; Irradiate Oil, a group in which the animals received olive oil and were irradiated with a single dose of 15 Gy of gamma radiation in the head and neck region; Irradiate, in which the animals were irradiated with 15 Gy single dose of gamma radiation; Vitamin E, a group in which the animals received solution of alpha-tocopherol acetate (vitamin E) but were not irradiated and Vitamin E irradiated, in which the animals received the solution of alpha-tocopherol acetate (vitamin E) before being irradiated with 15 Gy single dose of gamma radiation in the same region. Eight hours and thirty days after irradiation, the animals were anesthetized and the parotid glands of right and left sides were removed surgically. After surgical excision, the animals were sacrificed by anesthetic depth. The specimens were prepared, including in historesin and semi-series cuts were obtained in accordance with the protocol established by the discipline of Histology, Faculty of Dentistry of Piracicaba - UNICAMP. From each block were obtained three histologic slides, alternating with four cuts, with an interval of 30 cuts between them. The sections were mounted on slides, colored with hematoxylin and eosin, and then used for morphometric assessment. The data were submitted to analysis of variance (ANOVA) and Tukey test. Morphometric analysis of acinus in time of 8 hours showed statistically significant difference between the groups Vitamin E Irradiate and Oil Irradiate. At time of 30 days, the vitamin E Irradiate group and the Irradiate group, showed significant reduction in the amount of acinus compared to the Oil group. When the groups were evaluated in terms of time, there was significant reduction in the groups Oil Irradiate and Irradiate to 30 days. The morphometric analysis of interim and striated ducts showed no statistically significant differences between groups in both periods studied. Within the experimental conditions used, it is concluded that vitamin E showed mild radioprotective action in parotid glands of rats irradiated

ASSUNTO(S)

ionizing radiation radiação ionizante

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