Avaliação de diferentes fontes de nitrogênio em explantes de Cryptomeria japonica D.Don. "elegans" cultivados in vitro: análises bioquímicas e relações entre reguladores vegetais. / Evaluation of the different nitrate and ammonium concentrations in explants of cryptomeria japonica d. don. "elegans" cultivated in vitro .

AUTOR(ES)
DATA DE PUBLICAÇÃO

2002

RESUMO

Cryptomeria japonica D. Don. "elegans" is a fast-growing tree belonging to the Taxodiaceae and it is considered a responsive species in vitro . The aim of this work was to evaluate the effect of ten different concentrations of nitrate and ammonium in morphogenesis in vitro of Cryptomeria japonica D. Don. "elegans" explants. Analysis of relative growth rate, total soluble proteins, total soluble aminoacids, total non structural carbohydrates and electrophoresis of total proteins was performed in order to observe the role of different nitrogen sources on the growth and development of the explants cultivated in vitro . The concentratios of nitrate and ammonium that showed the best results were selected and used in an experiment with different concentrations of NAA and BAP to observe the shoot production. Each experiment was analised at the 30 th , 60 th and 90 th days of culture. The biochemical analysis was performed only at 90 th day of cultive. Concentrations from 26 to 31mmol.L -1 of NO3 - and lower than 5mmol.L -1 of NH4 + were the most effective for growth and development of the explants. However, the experiment with different concentrations of NNA and BAP showed that the best shoot production was achieved on 22,5mmol.L -1 of NO3- + 2,5mmol.L -1 of NH4 + and on 25,0mmol.L -1 of NO3 - + 5,0mmol.L -1 of NH4 + and with 2,0mg.L -1 of BAP + 1,0mg.L -1 of NNA.

ASSUNTO(S)

plants (physiology) culture cultivated vegetal biochemist reguladores vegetal bioquímica vegetal regulators plantas cultivadas (fisiologia) vegetal vegetal propagation propagação vegetal coníferas vegetal fabric conifers cultura de tecidos vegetais

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