Avaliação de componentes da matriz extracelular na reparação de defeitos de furca classe II após o enxerto de tecido reparativo de alvéolos dentários tratados com fatores de crescimento / Histological study of the healing of class II furcation defects following the graft of healing tissue from extraction sockets previously treated with growth factors.

AUTOR(ES)

Fernando Peixoto Soares

DATA DE PUBLICAÇÃO

2009

RESUMO

The tissue from healing extraction sockets is considered an excellent bone grafting material in the therapy of periodontal defects and the association of growth factors to the extraction sockets would increase the regenerative potential of this tissue when utilized as a bone graft. The aim of this investigation was to evaluate qualitatively the healing of acute class II furcation defects when grafted with granulation tissue from healing extraction sockets previously treated with an association of platelet derived growth factor-BB (PDGF-BB) and insulin-like growth factor-I (IGF-I), both in a concentration of 6 !g/ml. The second an third upper premolars of four dogs were extracted and the growth factors were applied into the sockets. After a healing period of five days, 24 class II furcation defects (12 tests and 12 controls) were surgically created at the buccal surface of the second, third and fourth mandibular premolars. The 12 experimental sites were grafted with the tissue from the healing sockets, while the 12 control sites healed without any graft. The flaps were coronally positioned and sutured. After 45 days, tissues were analyzed by immunohistochemistry for osteopontina (OPN), bone sialoprotein (BSP) and osteonectina (ONC), in a buccal-lingual plane. Histologically, healing occurred similarly in both groups. Pristine periodontal tissues extracellular matrix stained faintly for the tested antibodies, which characterized the presence of mature tissues. The staining of test and control healing tissues showed a more intense metabolic activity of the control group. This fact suggests that the tissues in the treated defect of test group were already in a more advanced phase of the repair process.

ASSUNTO(S)

bone transplant sialoproteína óssea insulin-like growth factor osteopontina fator de crescimento derivado de plaquetas-bb fator de crescimento semelhante à insulina-i enxerto ósseo osteonectina platelet derived growth factor

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