"Avaliação da desinfecção da dentina radicular em profundidade provocada pela irradiação do laser de diodo de alta potência" / Evaluation of root canal dentin desinfection in depth caused by high power diode laser irradiation.

AUTOR(ES)
DATA DE PUBLICAÇÃO

2007

RESUMO

The main cause for failure in Endodontics is the presence of microorganisms in root canal system. Enterococcus faecalis is a resistant microorganism presents in failure endodontic treatment. Therefore the disinfection plays fundamental rule in endodontic therapy. The laser irradiation presents properties that may improve the treatment success rates, reducing the microbial contamination. The aim of this study is evaluate the disinfection degree in depth caused by diode laser irradiation after chemomechanical procedures in vitro. Thirty extracted dental roots were sterilized by autoclave, inoculated by strain of Enterococcus faecalis ATCC 29212 during 4 weeks, and then randomly divided in 3 groups. Group 1: 10 specimens were instrumented by rotary files K3 (Sybron Endo, Orange, CA, USA) with 0.5 % sodium hypochlorite and urea peroxide (Endo PTC cream, Oficinalis Farmácia de Manipulação, São Paulo, SP, Brazil). At the end irrigation by 17 % EDTA-T was performed. The specimens were irradiated intracanal by diode laser 830 nm wavelength 3 W during 5 seconds, four times. Group 2: same procedures of group 1 without laser irradiation. Group 3: irrigation by saline solution. The specimens were divided in three thirds (apical, middle and cervical). Samples of dentin were obtained with carbide burs from inner canal walls of each third. The samples were immersed in 1 mL of peptonic water, and then aliquots were sowed to count viable cells in mEnterococcus agar (Difco?, Becton Drive, NJ, USA). The groups showed the following means: Group 1 ? 0, Group 2 ? 2,77 X 102 and Group 3 ? 171 X 102 CFU/mL. The disinfection degree achieved was 100 % in group 1, 98,39 % in group 2 both compared with group 3. The Kruskal-Wallis statistical analysis with Dunn method of comparison shows better results in group with diode laser irradiation (p<0.05), followed by group 2 and 3. Irradiation by diode laser in that samples and parameters achieved disinfection improvement in root dentin depth.

ASSUNTO(S)

hipoclorito de sódio endodontia enterococcus faecalis sodium hypochlorite enterococcus faecalis desinfecção endodontics diode laser laser de diodo desinfection

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