Avaliação comparativa da citotoxicidade in vitro dos solventes utilizados no retratamento endodôntico / Comparative evaluation in vitro of solvents citotoxicity used in endodontic retratament

AUTOR(ES)
DATA DE PUBLICAÇÃO

2003

RESUMO

SUMMARY The aim of this study was to compare in vitro solvents used in endodontic retreatment therapy: orange oil, eucalyptol, xylene, chloroform and halothane through cytotoxicity evaluation of each solvent in different final concentration and contact conditions to NIH 3T3 fibroblasts cells lines. The experiment was divided in four stages: stage 1- the solvents were mixed in culture medium (DMEM) in final concentrations of 0, 5, 10, 25, 50 and 100% placed on the cells in culture for periods of 5 minutes; stage 2- the solvents were mixed in culture medium in finals concentrations of 0, 10 and 25% placed on the cells in culture for periods of 10 and 15 minutes; stage 3a- the solvents were placed on the fibroblasts cells (direct contact) and added culture medium; stage 3b- the fibroblasts cells get first culture medium and after that solvents were placed (indirect contact), in bouth the finals concentrations were 0, 5 and 10% for periods of 5 minutes; stage 4- solvents were previously solubilized in absolut alcohol in 1:1 proportion and mixed in culture medium in 5% final concentration for periods of 5 minutes. The evaluation were achieved by cellular counting through dyed exclusion method-Trypan blue. The results showed that all solvents were toxic in vitro, and orange oil was less toxic and it allowed some cellular viability. And we could observe that a previous solvents preparation influenced in the viable cells percentage.

ASSUNTO(S)

solvents citotoxicity comparative evaluation avaliação comparativa endodontics citotoxicidade solventes endodontia

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