Autogenous control of PspF, a constitutively active enhancer-binding protein of Escherichia coli.

AUTOR(ES)

Jovanovic, G

RESUMO

Escherichia coli sigma54-dependent phage shock protein operon (pspA to -E) transcription is under the control of PspF, a constitutively active activator. Sigma70-dependent transcription of pspF is under autogenous control by wild-type PspF but not by a DNA-binding mutant, PspF deltaHTH. Negative autoregulation of PspF is continual and not affected by stimuli, like f1 pIV, that induce the pspA to -E operon. PspF production is independent of PspA (the negative regulator of the pspA to -E operon) and of PspB and -C (positive regulators).

Documentos Relacionados

• Identification, nucleotide sequence, and characterization of PspF, the transcriptional activator of the Escherichia coli stress-induced psp operon.
• New B-lymphocyte-specific enhancer-binding protein.
• Purification of the c-fos enhancer-binding protein.
• A functional assay for enhancer-binding proteins.
• A protein-induced DNA bend increases the specificity of a prokaryotic enhancer-binding protein