Atividade antinociceptiva, antipirÃtica e antiinflamatÃria do extrato bruto e do Ãcido fumarprotocetrÃrico isolado de Cladonia verticillaris (lÃquen)

AUTOR(ES)
DATA DE PUBLICAÇÃO

2003

RESUMO

The purpose of this work was to evaluate the anti-inflammatory, antinociceptive and antipyretic activity of the acetonic extract and the isolated fumarprotocetraric acid from Cladonia verticillaris (Raddi) Fr. ocurring on sandy soils of savannah-like vegetation of Mamanguape county (ParaÃba-Brazil). The acetonic extract was obtained from the dried lichen thallus and its composition was analyzed by thin layer chromatography (TLC) that showed the presence of the fumarprotocetraric acid, protocetraric acid, atranorin and other non-identified compounds. The fumarprotocetraric acid was isolated, purified and tested at several experimental models of inflammation, nociception and pyrexia. The results demonstrated significant (p<0,05) anti-inflammatory activity for the acetonic extract (200 mg/kg; p.o.) on acute inflammation induced by carrageenan at 30 (20,18%) and 180 (28,85%) minutes or dextran at 60 (38,27%), 120 (42,12%) and 180 (50,95%) minutes and on the chronic inflammation induced by formaldehyde from day 2 to day 10 (32,68%, 48,10%, 66,55%, 68,96%, 66,53%, 69,25%, 62,89%, 61,12% and 56,23%, respectively). To the antinociceptive tests, the extract (100 or 200 mg/kg; p.o.) showed significant (p<0,05) peripheral analgesic effect inhibiting the number of abdominal contortions (42,14% or 58,57%, respectively). The extract (200 mg/kg; p.o.) demonstrated significant (p<0,05) antipyretic activity on the fever induced by lipopolysaccharide at 30 (72,82%) and 60 (53,58%) minutes, respectively. The acetonic extract didnât show activity on the cotton pellet â induced granuloma and hot-plate test. The extract presented low toxicity, with no death until oral administration of the compound at 2g/kg; p.o. On the gastric toxicity test, the examinated stomachs didnât show lesions. The purified fumarprotocetraric acid (25 or 50 mg/kg; i.p.) presented significant (p<0,01) anti-edematogenic action on zymosan-induced paw edema at 60 (48,23% or 55,57%), 120 (46,84% or 56,24%), 180 (49,94% or 60,61%) and 240 (47,77% or 60,45%) minutes, respectively. To the antinociceptive tests, the fumarprotocetraric acid (400 mg/kg; p.o.) showed significant (p<0,01) peripheral analgesic effect inhibiting the number of abdominal contortions (60%). On fever induced by lipopolysaccharide (LPS), the fumarprotocetraric acid (25 or 50 mg/kg; i.p.) demonstrated significant (p<0,05) antipyretic activity at 30 (44,44% or 72,22%) and 60 (39,02% or 56,09%) minutes, respectively. The results suggest the acetonic extract obtained from C. verticillaris possess notable anti-inflammatory, antinociceptive and antipyretic activity and its major compound, the fumarprotocetraric acid, may be suggested to be the active principle responsible for those activities

ASSUNTO(S)

atividade antinociceptiva atividade antipirÃtica anti-inflammatory activity cladonia verticillaris antinociceptive activity Ãcido fumarprotocetrÃrico cladonia verticillaris atividade antiinflamatÃria liquens ciencias biologicas antipyretic activity fumarprotocetraric acid lichens

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