ASSAY OF T3 PHAGE BY PLAQUE COUNT

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Werly, Emil F. (Midwest Research Institute, Kansas City, Mo.), and Anne Monley. Assay of T3 phage by plaque count. J. Bacteriol. 87:1177–1179. 1964.—T3 phage-count determinations were made by the agar layer method, with various media being used for serial dilutions, plate media, and overlayer media. Plaques produced with Tryptose-phosphate-dextrose hard agar as plate and overlayer media were small, distinct, sharply defined, and easily counted. With the media described, several hundred plaques per plate can be counted, after either 4 or 20 hr of incubation.

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