As células linhagem negativa (Lin) de medula óssea atenuam a progressão da doença renal crônica / Lineage negative bone marrow cells attenuate the progression of chronic renal failure

AUTOR(ES)
DATA DE PUBLICAÇÃO

2008

RESUMO

Progressive renal failure continues to be a challenge. The use of bone marrowderived stem cells (SCs) represents a means of meeting that challenge. We used lineage-negative (Lin-) SCs to test the hypothesis that Lin- cell infusion decreases renal injury. Syngeneic Fischer 344 rats were submitted to 5/6 nephrectomy and divided into 3 groups: Nx (untreated); NxSC1 (receiving 2 × 106 Lin- cells on postnephrectomy day 15); and NxSC3 (receiving 2 × 106 Lin- cells on postnephrectomy days 15, 30 and 45). Controls were unoperated/untreated. On postnephrectomy day 60, clearance studies, immunohistochemistry and immunoblotting were performed. Lin- cell infusion effectively reduced postnephrectomy proteinuria, glomerulosclerosis, anemia, renal infiltration of immune cells and monocyte chemoattractant protein-1 protein expression, as well as decreasing the interstitial area. Immunostaining for proliferating cell nuclear antigen showed that, in comparison with controls, Nx rats presented greater cell proliferation, whereas NxSC1 rats and NxSC3 rats presented less cell proliferation than did Nx rats. Protein expression of p21 and VEGF increased after nephrectomy and decreased after Lin- cell infusion. Protein expression of eNOS reduced after nephrectomy and increased after cell infusion. These data suggest that SC treatment ameliorates progressive end-stage renal disease.

ASSUNTO(S)

linfócitos lymphocytes nitric oxide synthase Óxido nítrico sintase células tronco proteínas proto-oncogências p21 stem cells ratos endogâmicos f344 vascular endothelial growth factor insuficiência renal crônica fator de crescimento do endotélio vascular proto-oncogene proteins p21 (ras) macrófagos macrophages rats inbread f344 renal insufficiency chronic

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