A family of positive regulators related to the Pseudomonas putida TOL plasmid XylS and the Escherichia coli AraC activators.

AUTOR(ES)

Ramos, J L

RESUMO

The XylS family consists of a least 8 different transcriptional regulators. Six of these proteins are positive regulators for the catabolism of carbon sources (benzoate and sugars) in Escherichia coli, Pseudomonas putida and Erwinia carotovora, and two of them are involved in pathogenesis in Escherichia coli and Yersinia enterocolitica. Based on protein alignments, the members of this family exhibit a long stretch of homology at the C-terminal end. The regulators involved in the catabolism of carbon sources stimulate transcription from their respectively regulated promoters only in the presence of effectors. In two of the regulators, mutations at the non-homologous N-terminus alter affinity and specificity for effectors while mutations at the conserved C-terminus part decrease activation of transcription from their corresponding regulated promoters. It is thus probable that the variable N-terminus end in this family of regulators contains the motif involved in effector recognition, while the C-terminal end is involved in DNA-binding. These proteins seem to be related by common ancestry and may act through similar mechanisms of positive regulation effected through similar folding patterns.

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