Anticorpos anti-Stx como ferramentas na detecção de Escherichia coli produtora da toxina de Shiga (STEC). / Antibodies anti-Stx as tools on detection of Shiga toxin-producing Escherichia coli (STEC).

AUTOR(ES)
DATA DE PUBLICAÇÃO

2008

RESUMO

STEC infection is a public health problem, causing hemolytic-uremic syndrome and haemorrhagic colitis. The detection of STEC isolates depends on a sensitive, specific and low cost diagnostic method. Thus, the aim of this study was to develop a capture ELISA using polyclonal and monoclonal antibodies. For this purpose, first of all a growth media that induce better expression and production of Stx was defined and monoclonals anti-Stx1 e anti-Stx2 antibodies were produced. The results show that the growth of the isolates for 4hs in EC broth with ciprofloxacin increased the production of Stx in the bacterial supernantant, and the monoclonal anti-Stx1antibody produced was able to recognize the A subunit of both toxins, besides showing higher affinity constant than anti-Stx2. Thus, the capture ELISA was standardized using 250 mg/ml of the IgG enriched fraction of rabbit anti-Stx1 sera and anti-Stx2 for coating and 2,5 mg/ml of monoclonal anti-Stx1 antibody for the capture of the toxin, showing 80,7% of sensibility and e 100% de specificity.

ASSUNTO(S)

diagnóstico escherichia coli bacteriologia aplicada escherichia coli diagnosis citotoxinas citotoxins monoclonals antibodies applied bacteriology elisa anticorpos monoclonais elisa

ACESSO AO ARTIGO

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