Anticodon conformation and accessibility in wild-type and suppressor tryptophan tRNA from E. coli.
AUTOR(ES)
Buckingham, R H
RESUMO
The association between Trp-tRNA and Pro-tRNA, which have complementary anticodon sequences, has been used as a probe of anticodon conformation. It is unaffected, however, by the base change in the D-stem present in UGA-suppressor Trp-tRNA. This does not support the hypothesis that UGA suppression depends upon a conformational change induced in the anticodon. The stable denatured form of wild-type Trp-tRNA no longer interacts with Pro-tRNA; the structure of the anticodon region must therefore be quite different in the denatured form.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=342958Documentos Relacionados
- Expression of the ogt gene in wild-type and ada mutants of E. coli.
- Comparative mutational analysis of wild-type and stretched tRNA3(Leu) gene promoters.
- Identity determinants of E. coli tryptophan tRNA.
- Incorporation of excess wild-type and mutant tRNA(3Lys) into human immunodeficiency virus type 1.
- Gene replacement with linear DNA in electroporated wild-type Escherichia coli.