Analysis and chromosomal mapping of Leishmania (Leishmania) amazonensis amastigote expressed sequence tags

AUTOR(ES)

Gentil, Luciana Girotto, Lasakosvitsch, Fernanda, Silveira, José Franco da, Santos, Márcia Regina Machado dos, Barbiéri, Clara Lúcia

FONTE

Memórias do Instituto Oswaldo Cruz

DATA DE PUBLICAÇÃO

05/07/2007

RESUMO

The characterization of expressed sequence tags (ESTs) generated from a cDNA library of Leishmania (Leishmania) amazonensis amastigotes is described. The sequencing of 93 clones generated new L. (L.) amazonensis ESTs from which 32% are not related to any other sequences in database and 68% presented significant similarities to known genes. The chromosome localization of some L. (L.) amazonensis ESTs was also determined in L. (L.) amazonensis and L. (L.) major. The characterization of these ESTs is suitable for the genome physical mapping, as well as for the identification of genes encoding cysteine proteinases implicated with protective immune responses in leishmaniasis.

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