Análise proteômica das salivas dos triatomíneos Rhodnuis brethesi, Rhodnius robustus e Panstrongylus megistus, vetores da doença de Chagas

AUTOR(ES)

Camila Miranda Costa

DATA DE PUBLICAÇÃO

2009

RESUMO

Triatomine bugs acting as vectors of Chagas‟ disease are haematophagous organisms in all of its evolvement phases. Their feeding success is greatly related to their salivary glands content. The presence of a pool of specific proteins allows this insect to access its food by counteracting host haemostatic mechanisms, such as platelet aggregation, clotting and vasoconstriction, as well as host inflammatory reactions. Since these components are relevant in controlling the Trypanosoma cruzi transmission process, the salivas of epidemiologically significant triatomine bugs in Brazil, Panstrongylus megistus at Cerrado and Atlantic Rainforest Biomas, and Rhodnius brethesi and Rhodnius robustus at Amazon region, were harvested and submitted to proteomic research. Initially, the experimental conditions for wide-pH-ranged separation by two-dimensional electrophoresis (2DE) separation at wide pH range were optimized. The average number of spots of each salivary profile varied from 129 to 320. Comparative analysis of all two-dimensional maps revealed divergence among genera. The identification of the obtained protein spots from such maps was made by peptide mass fingerprinting, peptide fragmentation and de novo sequencing using MALDI-TOF/TOF mass spectrometers. The data revealed largely anti-haemostatic profiles for Rhodnius salivas, which presented mainly lipocalins, potential anti-platelet aggregation proteins. Proteomic analysis of Panstrongylus megistus saliva is still in progress and the first results imply the presence of potentially anti-platelet aggregation proteins too. Salivary proteomes of these triatominae vectors appear to be rich in platelet aggregation inhibitors which promote the blood feeding of these insects. Future analysis of these and other salivary spots will permit better understanding of vector-host interaction and lead to the possibility of new findings on pharmacologically active molecules useful for haemopathy treatments.

ASSUNTO(S)

biologia molecular doença de chagas triatomíne proteome triatomíneo of chagas disease proteoma

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