Analise genotipica e mutacinotipagem de Streptococcus mutans isolados de voluntarios carie-ativos e livres de carie

AUTOR(ES)

Regianne Umeko Kamiya

DATA DE PUBLICAÇÃO

2003

RESUMO

The objectives of this study were: to compare the activity of mutacin production, the fenotypic/genotypic diversity of S. mutans isolated from caries active and caries free individuals, and to evaluate the association index and the power discriminatory of Mutacin Typing and AP-PCR. In the present study, 319 strains of S. mutans isolated from saliva, dental plaque and tongue dorsum of 8 caries active and 8 caries free voluntareers were submitted to Mutacin Typing and AP-PCR. The mutacinogeny profile was traced, using 13 indicator strains between them: S. mutans, S. sobrinus, S. mitis, S. salivarius, S. sanguinis, S. oralis e S. cricetus and the genetic polymorphism was detected with primers OPA-02 and OPA-13. Mutacin was produced by 79,62% out of 319 isolates (245/319) inhibiting one or more of the 13 indicator strains. No corellation was found between the S. mutans fenotypic/genotypic diversity and caries activity, by the typing methods employed. The Mutacin typing allowed to evidence 55 distinct fenotypic patterns whereas AP-PCR detected 101 distinct patterns genotypic. The Mutacin Typing was limited to analyze S. mutans heterogeneity intra individually. The association index mean between Mutacin Typing and AP-PCR was 77,38%, and the discriminatory power of AP-PCR (0,975) was greater than the one Mutacin Typing (0,925). Bacteriocin Typing was limited to study for fenotypic diversity, however it was possible to demonstrate that S. mutans isolated from caries active group showed higher inhibitory activity than S. mutans isolated from caries free group, and the last presented highest antagonic effect against S. sanguinis, S. oralis and S. mitis (the predominant colonizers of this population). Theorically, inhibiting potencial competitors, S. mutans would have succesful colonization, either by nutricional or adhesion receptor aquisitions

ASSUNTO(S)

streptococcus mutans caries dentarias

Documentos Relacionados

• Avaliação do padrão de clonalidade e virulencia de S. mutans isolados de individuos livres de carie e carie-ativos
• Detecção do polimorfismo genetico de Streptococcus mutans isolados de individuos livres de carie (CPOD=0)
• Polimorfismo genetico de Streptococcus mutans isolados da cavidade oral de individuos com lesões de carie coronaria e radicular
• Enzimotipagem e genotipagem de isolados de Candida albicans da cavidade oral de crianças carie ativas e livres de carie
• Atividade do carvacrol e timol in vivo na inibiÃÃo de streptococcus mutans e experiÃncia de cÃrie em crianÃas livres de cÃrie: um estudo longitudinal