Análise da expressão de regiões da proteína Circumsporozoíta de Plasmodium sp. em Aedes aegypti infectado por Plasmodium gallinaceum. / Expression analyses of Plasmodium sp. Circumsporozoite protein regions in Plasmodium gallinaceum infected Aedes aegypti.

AUTOR(ES)
DATA DE PUBLICAÇÃO

2009

RESUMO

Transgenic mosquitoes that impair malaria transmission can be an alternative control but currently an effective line is not available. A better understanding of mosquito interaction with pathogens is very important to improve refractory transgene design. Circumsporozoite protein (CSP) has two conserved domains that could be involved in spz penetration into mosquito salivary glands. Our aim was to express peptides encompassing these conserved regions in the mosquito hemolymph using Sindbis virus transient expression system and transgenesis technology. If CSP is involved in this process these peptides will compete with sporozoites impairing its penetration. Five Sindbis virus and four transgenic lines were constructed and challenged with P. gallinaceum. Our results showed these peptides could not impair sporozoites penetration in salivary glands, mainly because the recombinant proteins could not be produced or detected. Improving transgene design using codon usage and other technologies will be essential for expressing foreign proteins in transgenic mosquitoes.

ASSUNTO(S)

plasmodium mosquitos aedes mosquitoes aedes plasmodium transgenes transgenes proteínas (análise) protein (analysis)

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