An octamer of histones H3 and H4 forms a compact complex with DNA of nucleosome size.

AUTOR(ES)

Simon, R H

RESUMO

Equimolar mixtures of histones H3 and H4 have been reconstituted onto DNA of nucleosome core size. Two distinct complexes are formed in a relative abundance that depends on the starting ratio of H3 + H4 to DNA. One of these complexes contains two H3-H4 dimers for each DNA molecule, and has a sedimentation coefficient of 7.5S. The other complex contains an octamer consisting of four H3-H4 dimers, and has a sedimentation coefficient of 10.4S. On the basis of these measurements, we conclude that the octamer complex (but not the tetramer complex) is a fully folded, compact structure resembling the nucleosome.

Documentos Relacionados

• Histones H3 and H4 interact with the ends of nucleosome DNA.
• A nucleosome-like structure containing DNA and the arginine-rich histones H3 and H4.
• Heritable chromatin structure: Mapping “memory” in histones H3 and H4
• H3 and H4 histone cDNA sequences from Xenopus: a sequence comparison of H4 genes.
• The N-terminal domains of histones H3 and H4 are not necessary for chromatin assembly factor-1- mediated nucleosome assembly onto replicated DNA in vitro