An evaluation of p16INK4a expression in cervical intraepithelial neoplasia specimens, including women with HIV-1
AUTOR(ES)
Nicol, Alcina F, Golub, Jonathan E, Silva, José R Lapa e, Cunha, Cynthia B, Amaro-Filho, Sergio M, Oliveira, Nathalia S, Menezes, Willker, Andrade, Cecília Viana, Russomano, Fabio, Tristão, Aparecida, Grinsztejn, Beatriz, Friedman, Ruth Khalili, Oliveira, Marcia P, Pires, Andrea, Nuovo, Gerard J
FONTE
Memórias do Instituto Oswaldo Cruz
DATA DE PUBLICAÇÃO
2012-08
RESUMO
Although several studies have evaluated the role of p16INK4a as a diagnostic marker of cervical intraepithelial neoplasia (CIN) and its association with disease progression, studies regarding the role of p16INK4a in human immunodeficiency virus (HIV)-infected patients remain scarce. The present study was designed to determine the potential utility of p16INK4a as a diagnostic marker for CIN and invasive cervical cancer in HIV-positive and negative cervical specimens. An immunohistochemical analysis of p16INK4a was performed in 326 cervical tissue microarray specimens. Performance indicators were calculated and compared using receiving operating characteristics curve (ROC)/area under the curve. In HIV-1-negative women, the percentage of cells that was positive for p16INK4a expression was significantly correlated with the severity of CIN (p < 0.0001). A ROC curve with a cut-off value of 55.28% resulted in a sensitivity of 89%, a specificity of 81%, a positive predictive value of 91% and a negative predictive value of 78%. HIV-seropositive women exhibited decreased expression of p16INK4a in CIN2-3 specimens compared with HIV-negative specimens (p = 0.031). The ROC data underscore the potential utility of p16INK4a under defined conditions as a diagnostic marker for CIN 2-3 staging and invasive cervical cancer. HIV-1 infection, however, is associated with relatively reduced p16INK4a expression in CIN 2-3.
Documentos Relacionados
- Expression of p16INK4a and of p53 as prognostic markers of cervical intraepithelial neoplasia and their relationship with high risk human papillomavirus
- Análise de polimorfismos e de expressão do gene p16INK4a em neoplasias cervicais
- Obligate Roles for p16Ink4a and p19Arf-p53 in the Suppression of Murine Pancreatic Neoplasia
- JunB suppresses cell proliferation by transcriptional activation of p16INK4a expression
- p16Ink4a Interferes with Abelson Virus Transformation by Enhancing Apoptosis