Amylase production by endophytic fungi Cylindrocephalum sp. isolated from medicinal plant Alpinia calcarata (Haw.) Roscoe
AUTOR(ES)
Sunitha., V. H., Ramesha., A., Savitha., J., Srinivas., C
FONTE
Braz. J. Microbiol.
DATA DE PUBLICAÇÃO
2012-09
RESUMO
Amylases are among the most important enzymes used in modern biotechnology particularly in the process involving starch hydrolysis. Fungal amylase has large applications in food and pharmaceutical industries. Considering these facts, endophytic fungi isolated from the plant Alpinia calcarata (Haw.) Roscoe were screened for amylolytic activity on glucose yeast extract peptone agar (GYP) medium. Among thirty isolates of endophytic fungi, isolate number seven identified as Cylindrocephalum sp. (Ac-7) showed highest amylolytic activity and was taken for further study. Influence of various physical and chemical factors such as pH, temperature, carbon and nitrogen sources on amylase production in liquid media were studied. The maximal amylase production was found to be at 30ºC and at pH 7.0 of the growth medium. Among the various carbon and nitrogen sources tested, maltose at 1.5% and Sodium nitrate at 0.3% respectively gave optimum amylase production.
Documentos Relacionados
- Endophytic fungi from medicinal plant Bauhinia forficata: Diversity and biotechnological potential
- Bioactive endophytic fungi isolated from Caesalpinia echinata Lam. (Brazilwood) and identification of beauvericin as a trypanocidal metabolite from Fusarium sp.
- Endophytic Colonization and In Planta Nitrogen Fixation by a Herbaspirillum sp. Isolated from Wild Rice Species
- Production of α-amylase from Streptomyces sp. SLBA-08 strain using agro-industrial by-products
- Optimization of zofimarin production by an endophytic fungus, Xylaria sp. Acra L38