Ammonia Assimilation in Alnus glutinosa and Glycine max: SHORT-TERM STUDIES USING [13N]AMMONIUM 1
AUTOR(ES)
Schubert, Karel R.
RESUMO
The pattern of assimilation of NH4+ by Alnus glutinosa, a N2-fixing, nonleguminous angiosperm, was examined. Detached nodules, roots, and nodulated roots of intact plants were exposed to 13NH4+ for up to 15 minutes. Glutamine was the most highly labeled compound at all times; the only other compound labeled significantly was glutamate. Similar results were obtained after incubating soybean (L. merr) nodules and roots with 13NH4+. These observations and the results of pulse-labeling and inhibitor studies with nodules of Alnus were distinctly different from those predicted for the assimilation of NH4+ via glutamine synthetase and glutamate synthase and suggest that glutamate dehydrogenase may play a major role in the assimilation of exogenously supplied NH4+.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=425750Documentos Relacionados
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