Acetone and Butanol Production by Clostridium acetobutylicum in a Synthetic Medium
AUTOR(ES)
Monot, Frédéric
RESUMO
The effect of the component concentrations of a synthetic medium on acetone and butanol fermentation by Clostridium acetobutylicum ATCC 824 was investigated. Cell growth was dependent on the presence of Mg, Fe, and K in the medium. Mg and Mn had deleterious effects when in excess. Ammonium acetate in excess caused acid fermentation. The metabolism was composed of two phases: an acid phase and a solvent one. Low concentrations of glucose allowed the first phase only. The theoretical ratio of the conversion of glucose to solvents, which was 28 to 33%, was obtained with the following medium: MgSO4, 50 to 200 mg/liter; MnSO4, 0 to 20 mg/liter; KCl, 0.015 to 8 g/liter (an equivalent concentration of K+ was supplied in the form of KH2PO4 and K2HPO4); FeSO4, 1 to 50 mg/liter; ammonium acetate, 1.1 to 2.2 g/liter; para-aminobenzoic acid, 1 mg/liter; biotin, 0.01 mg/liter; glucose, 20 to 60 g/liter.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=242190Documentos Relacionados
- Butanol Production by a Butanol-Tolerant Strain of Clostridium acetobutylicum in Extruded Corn Broth
- Acetone, Isopropanol, and Butanol Production by Clostridium beijerinckii (syn. Clostridium butylicum) and Clostridium aurantibutyricum
- Regulation of the sol Locus Genes for Butanol and Acetone Formation in Clostridium acetobutylicum ATCC 824 by a Putative Transcriptional Repressor
- Control of Butanol Formation in Clostridium acetobutylicum by Transcriptional Activation
- Carboxymethyl cellulase and cellobiase production by Clostridium acetobutylicum in an industrial fermentation medium.
