Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes

AUTOR(ES)

Vandesompele, Jo

FONTE

BioMed Central

RESUMO

Using real-time reverse transcription PCR ten housekeeping genes from different abundance and functional classes in various human tissues were evaluated. The conventional use of a single gene for normalization leads to relatively large errors in a significant proportion of samples tested.

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