A rapid detection of multidrug-resistant Mycobacterium tuberculosis by a nitrate reductase assay on blood agar

AUTOR(ES)

Coban, Ahmet Yilmaz, Cayci, Yeliz Tanriverdi, Deveci, Aydin, Akgunes, Alper, Uzun, Meltem, Durupinar, Belma

FONTE

Memórias do Instituto Oswaldo Cruz

DATA DE PUBLICAÇÃO

2011-05

RESUMO

The susceptibility of 49 Mycobacterium tuberculosis clinical isolates to isoniazid (INH) and rifampisin (RIF) (28 multi-drug resistant-tuberculosis samples) was determined by a nitrate reductase assay (NRA) on blood agar. Agreement between the NRA and other testing methods was found to be 93.8% for both INH and RIF. The sensitivity, specificity, positive predictive value and negative predictive value for INH were 92.8%, 94.2%, 86.6% and 97%, respectively. The sensitivity, specificity, positive predictive value and negative predictive value for RIF were 90.4%, 96.4%, 95% and 93.1%. In conclusion, we show here that blood agar can be used effectively for the NRA test.

Documentos Relacionados

• Rapid detection of multidrug-resistant Mycobacterium tuberculosis using the malachite green decolourisation assay
• Nitrate reductase assay using sodium nitrate for rapid detection of multidrug resistant tuberculosis
• Rapid detection of multidrug-resistant Mycobacterium tuberculosis using the mycobacteria growth indicator tube (MGIT) system
• Multidrug-resistant tuberculosis
• Evaluation of four colourimetric susceptibility tests for the rapid detection of multidrug-resistant Mycobacterium tuberculosis isolates