A perfect functional marker for the gene of intermediate amylose content Wx-in in rice (Oryza sativa L.)
AUTOR(ES)
Zhou, Lei, Chen, Shaoyu, Yang, Guocai, Zha, Wenjun, Cai, Haiya, Li, Sanhe, Chen, Zhijun, Liu, Kai, Xu, Huashan, You, Aiqing
FONTE
Crop Breed. Appl. Biotechnol.
DATA DE PUBLICAÇÃO
2018-03
RESUMO
Abstract The amylose content (AC) of rice is a key determinant of the grain cooking and eating quality. The Waxy (Wx) gene, which encodes granule-bound starch synthase I (GBSSI), is the major gene controlling AC in rice. The A - C substitution in exon 6 (Ex6A/C) alters the amino acid codon from serine to tyrosine and is associated with intermediate-AC rice (Wx-in). In this paper, we reported a perfect functional marker for the SNP Ex6A/C of Wx-in based on polymerase chain reaction with confronting two-pair primers (PCR-CTPP) in a single-tube PCR assay. Specificity and applicability of the PCR assay were verified in a Chinese mini core collection (MCC) of Oryza sativa L. and a breeding population. Were commend the use of the simple, inexpensive assay for routine genotyping for intermediate AC in the breeding population or discrimination of Wx-in genotypes in rice germplasm.
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