A Calcium-Activated Phytase from Pollen of Lilium longiflorum1
AUTOR(ES)
Scott, Jonathan J.
RESUMO
A phytase was isolated and partially purified from the pollen of Lilium longiflorum Thumb. Optimum activity was at pH 8.0. The phytase was activated by Ca2+ and Sr2+ but not by the other divalent cations tested. Activity was inhibited by ethylenediaminetetraacetate. The phytase had a temperature optimum of 55 to 60°C and an activation energy of about 12,700 calories/mole. Extraction of L. longiflorum pollen with 0.1% Triton X-100 increased recovery of the phytase by nearly 4-fold. The phytase had a molecular weight of about 88,000 as determined by gel filtration chromatography and a Km value of 7.2 micromolar for phytic acid in the presence of Ca2+.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1056114Documentos Relacionados
- β-1, 3-Glucan Synthase from Lilium longiflorum Pollen 1
- A human intermediate conductance calcium-activated potassium channel
- Calcium-activated potassium channels in human platelets.
- Single calcium-activated potassium channels recorded from cultured rat sympathetic neurones.
- Calcium-activated potassium channels in goldfish hair cells.
