5S rDNA characterization in twelve Sciaenidae fish species (Teleostei, Perciformes): depicting gene diversity and molecular markers
AUTOR(ES)
Alves-Costa, Fernanda A., Martins, Cesar, Matos, Fernanda Del Campos de, Foresti, Fausto, Oliveira, Claudio, Wasko, Adriane P.
FONTE
Genetics and Molecular Biology
DATA DE PUBLICAÇÃO
2008
RESUMO
In order to extend the genetic data on the Sciaenidae fish family, the present study had the purpose to characterize PCR-generated 5S rDNA repeats of twelve species of this group through PAGE (Polyacrylamide Gel Electrophoresis) analysis. The results showed the occurrence of at least two different 5S rDNA size classes in all the species. Moreover, 5S rDNA repeats of one of the studied species - Isopisthus parvipinnis - were cloned and subjected to nucleotide sequencing and Southern blot membrane hybridization analyses, which permitted to confirm the existence of two major 5S rDNA classes. Phylogenetic analysis based on the nucleotide sequences of different 5S rDNA repeats of I. parvipinnis lead to their separation into two major clusters. These results may reflect the high dynamism that rules the evolution rate of 5S rDNA repeats. The obtained data suggest that 5S rDNA can be useful in genetic analyses to identify species-specific markers and determine relationships among species of the Sciaenidae group.
Documentos Relacionados
- Nucleotide sequence, genomic organization and chromosome localization of 5S rDNA in two species of Curimatidae (Teleostei, Characiformes)
- Chromosomal location of 18S and 5S rDNA sites in Triportheus fish species (Characiformes, Characidae)
- FISH-mapping of the 5S rDNA locus in chili peppers (Capsicum-Solanaceae)
- Heterochromatin diversity and its co-localization with 5S and 45S rDNA sites in chromosomes of four Maxillaria species (Orchidaceae)
- Discrimination of Shark species by simple PCR of 5S rDNA repeats