Aqueous Two Phase System
Mostrando 1-12 de 120 artigos, teses e dissertações.
-
1. BROMELAIN SEPARATION AND PURIFICATION PROCESSES FROM PINEAPPLE EXTRACT
Abstract The concern about the effective use of agro-industrial residues from the food industry has increased. Bromelain is an enzyme mainly obtained from the pineapple plant (A. comosus), including its parts normally considered as waste. It is a high value-added product with a wide variety of applications such as in food, cosmetic and pharmaceutical industr
Braz. J. Chem. Eng.. Publicado em: 30/09/2019
-
2. Liquid-liquid equilibria in aqueous two-phase ethanol/salt systems at different temperatures and their application to anthocyanins extraction
Abstract Aqueous two-phase systems (ATPS) are formed by mixtures of chemical species that, under certain conditions, separate into two immiscible phases, with water as the major component in global terms and, at lower concentrations, polymers, salts, ionic liquids and alcohols, depending on the system type. Different parameters influence ATPS equilibrium suc
Food Sci. Technol. Publicado em: 16/05/2019
-
3. Fragmental Method KowWIN as the Powerful Tool for Prediction of Chromatographic Behavior of Novel Bioactive Urea Derivatives
A series of the new urea derivatives with antinociceptive activity has been chromatographically evaluated using reversed phase materials: Zorbax Extend-C18, Cogent UDC Cholesterol with organic-aqueous eluent systems with two organic modifiers (methanol and acetonitrile). The chromatographic lipophilicity parameters: log kw, S and φ0 were determined basing o
J. Braz. Chem. Soc.. Publicado em: 2016-12
-
4. CHROMIUM EXTRACTION BY MICROEMULSIONS IN TWO- AND THREE-PHASE SYSTEMS
Abstract Microemulsion systems were used to remove chromium from an aqueous solution obtained from acid digestion of tannery sludge. The systems were composed by: coconut oil soap as surfactant, 1-butanol as cosurfactant, kerosene as the oil phase, and chromium solution as the aqueous phase. Two- and three-phase microemulsion extraction methods were investig
Braz. J. Chem. Eng.. Publicado em: 2015-12
-
5. PARTIÇÃO DE PROTEÍNAS DE SORO DE LEITE EM SISTEMAS AQUOSOS BIFÁSICOS BASEADOS EM LÍQUIDOS IÔNICOS
In this work, we used an aqueous two-phase system (ATPS) consisting of the ionic liquid [C4mim][Cl] and the salt K2HPO4 to partition α-lactalbumin, β-lactoglobulin, and lactoferrin whey proteins. Extraction efficiency values above 87% indicate that the proteins primarily migrated to the ionic liquid-rich phase (top phase). Partition coefficient values rang
Quím. Nova. Publicado em: 2015-11
-
6. Separation, Concentration and Determination of Trace Thiamphenicol in Egg, Milk and Honey Using Polyoxyethylene Lauryl Ether-Salt Aqueous Two-Phase System Coupled with High Performance Liquid Chromatography
As a pretreatment technique to trace analysis, polyoxyethylene lauryl ether (POELE10)- salt aqueous two-phase extraction system (ATPES) is coupled with high performance liquid chromatography to analyse thiamphenicol (TAP) in egg, milk and honey. The analysis of the phase behaviors of the ATPES found that the extraction efficiency and enrichment factor of TAP
J. Braz. Chem. Soc.. Publicado em: 2015-06
-
7. Purification of C-phycocyanin from Spirulina platensis in aqueous two-phase systems using an experimental design
C-phycocyanin from Spirulina platensis was purified in aqueous two-phase systems (ATPS) of polyethylene glycol (PEG)/potassium phosphate, varying the molar mass of the PEG. Results using a full factorial design showed that an increase in the concentration of salt and decrease in the concentration of PEG caused an increment in the purification factor for all
Braz. arch. biol. technol.. Publicado em: 2015-02
-
8. Influence of salts on the coexistence curve and protein partitioning in nonionic aqueous two-phase micellar systems
Aqueous two-phase micellar systems (ATPMS) can be exploited in separation science for the extraction/purification of desired biomolecules. Prior to phase separation the surfactant solution reaches a cloud point temperature, which is influenced by the presence of electrolytes. In this work, we provide an investigation on the cloud point behavior of the nonion
Braz. J. Chem. Eng.. Publicado em: 2014-12
-
9. Optimization of extraction and determination of chloramphenicol in livestock meat samples using aqueous two-phase system of n-propanol and potassium citrate coupled with HPLC
Um sistema de duas fases aquosas (ATPS) com base em álcool de cadeia curta e sal foi o método para a pré-concentração, separação, e análise de cloranfenicol (CAM), associado à cromatografia líquida de alta performance com detector de ultravioleta-visível (HPLC-UV). A fim de selecionar o ATPS adequado para a extração de CAM, diferentes ATPSs fora
J. Braz. Chem. Soc.. Publicado em: 2013-04
-
10. Extraction of lipase from Burkholderia cepacia by PEG/Phosphate ATPS and its biochemical characterization
This work aimed to study the partitioning of a lipase produced by Burkholderia cepacia in PEG/Phosphate aqueous two phase system (ATPS) and its characterization. Lipase was produced by B. cepacia strains in a fermenter. Enzyme partitioning occurred at pH 6.0 and 8.0, using PEG 1500 and 6000 on two tie lines. Metal ions, pH and temperature effects on enzyme a
Brazilian Archives of Biology and Technology. Publicado em: 2012-02
-
11. Partition of proteases from Lentinus citrinus DPUA 1535 by the Peg/Phosphate Aqueous Two-Phase System
A full two-level factorial design was employed to study the influence of PEG molar mass (MM PEG), PEG concentration (C PEG) and phosphate concentration (C PHOSPH) on proteases partition by Lentinus citrinus DPUA 1535 in a PEG/phosphate aqueous two-phase system (ATPS). For all ATPS studied, proteases partitioned for the top phase and the best proteases extrac
Quím. Nova. Publicado em: 2012
-
12. Enfermidades Cutâneas Digitais Bovina: Aspectos Genéticos E Terapêuticos / Digital dermatitis disease bovine: genetic and therapeutic aspects
This study has aimed do evaluate a risk factor for bovine digital dermatitis and the efficacy of 5% aqueous barbatimão extract solution as ancillary in interdigital skin incisional wound repair. One hundred bovine females, 50 Holstein and 50 Girolando, from two different farms were used for the risk factor study. Experimental units were divided into four gr
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 18/03/2011