Agrobacteria
Mostrando 1-12 de 49 artigos, teses e dissertações.
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1. Aumento da transformação genética transiente em embriões imaturos do genótipo brasileiro de milho BR 451 co-cultivados com Agrobacterium tumefaciens
RESUMO. A engenharia genética ampliou as possibilidades do melhoramento genético vegetal, apoiando ideais de sustentabilidade. A Agrobacterium tumefaciens é o sistema de transformação preferido, uma vez que permite a produção de plantas transgênicas com maior estabilidade na expressão gênica e na herdabilidade. A Agrobactéria e o tecido vegetal de
Acta Sci., Agron.. Publicado em: 16/07/2018
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2. Transformação genética da variedade brasileira de milho BR 451 pelo método da Agrobacterium tumefaciens
RESUMO: A introdução de genes de forma assexual por meio da engenharia genética tem ampliado as possibilidades do melhoramento genético vegetal. No entanto, devido principalmente a baixa resposta in vitro, a transformação genética tem se limitado a poucos genótipos das espécies de interesse agronômico. Visando estabelecer protocolo de transformaç
Cienc. Rural. Publicado em: 09/10/2017
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3. Cultura de tecidos de feijoeiro visando a introducao de genes exogenos.
O objetivo deste trabalho foi determinar parametros da cultura de tecidos de feijoeiro necessarios para a aplicacao da tecnica de transformacao via Agrobacterium. Diversos genotipos de feijoeiro (Phaseolus vulgaris L.) foram cultivados in vitro. Alguns parametros visando a infeccao de apices caulinares com Agrobacterium tumefaciens foram otimizados, como a e
Pesquisa Agropecuaria Brasileira. Publicado em: 2011
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4. Importância da região amino-terminal da proteína RolA de Agrobacterium rhizogenes em sua atividade biológica.
A proteina RolA e originaria de Agrobacterium rhizogenes, uma bacteria fitopatogenica que provoca a doenca conhecida como raiz em cabeleira (?ghairy root?h). RolA atua no processo de infeccao, sendo codificada no T-DNA do plasmideo Ri da agrobacteria. Embora a expressao de RolA em plantas implique em severas alteracoes morfologicas e fisiologicas, seu mecani
Brasília: Embrapa Recursos Genéticos e Biotecnologia. Publicado em: 2011
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5. Identificação de genes expressos durante a fase pré-simbiótica da associação ectomicorrízica entre Hydnangium sp. e Eucalyptus grandis e transformação de fungos ectomicorrízicos / Identification of genes expressed during the pre-symbiotic interaction in the Hydnangium sp.-Eucalyptus grandis ectomycorrhizal association and transformation of ectomycorrhizal fungi
Gene expression in the ectomycorrhizal fungus Hydnangium sp. during the pre-symbiotic interaction was evaluated using an in vitro mycorrhization technique aiming at constructing a suppressive subtractive cDNA library. The fungus was cultivated in the presence of Eucalyptus grandis roots, with no direct physical contact between both organisms. Genes that code
Publicado em: 2008
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6. Morfogênese in vitro e transformação genética de citros mediada por Agrobacterium tumefaciens / Morphogenesis in vitro and Agrobacterium tumefaciens- mediated genetic transformation of citrus
Current protocols used for citrus genetic transformation have resulted in a low number of transgenic plants, furthermore, it used juvenile material which source of explants. Plants regenerated from these sources of explants have long juvenile periods for initial fruit production and are necessary many years for evaluation of the introduced characteristics of
Publicado em: 2008
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7. Attachment of Agrobacteria to Grape Cells †
The presence of the Ti plasmid favorably influences the attachment of agrobacteria to grape callus cells, especially during the early stages of a 2-h incubation. Agrobacterium strains attached to a similar extent to both the crown gall-resistant cultivar (Catawba), Vitis labruscana, and the crown gall-susceptible cultivar (Chancellor), Vitis sp. Attachment o
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8. Host range encoded by the Agrobacterium tumefaciens tumor-inducing plasmid pTiAg63 can be expanded by modification of its T-DNA oncogene complement.
Agrobacterium tumefaciens harboring pTiA6 incite unorganized tumors on Nicotiana rustica, sunflowers, carrots, and tomatoes, whereas isogenic strains of agrobacteria harboring pTiAg63 form "rooty" tumors on N. rustica and are essentially avirulent on sunflowers, carrots, and tomatoes. In this report we show that the different host range characteristics of th
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9. Novel Tellurite-Amended Media and Specific Chromosomal and Ti Plasmid Probes for Direct Analysis of Soil Populations of Agrobacterium Biovars 1 and 2
Ecology and biodiversity studies of Agrobacterium spp. require tools such as selective media and DNA probes. Tellurite was tested as a selective agent and a supplement of previously described media for agrobacteria. The known biodiversity within the genus was taken into account when the selectivity of K2TeO3 was analyzed and its potential for isolating Agrob
American Society for Microbiology.
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10. Seasonal Fluctuations and Long-Term Persistence of Pathogenic Populations of Agrobacterium spp. in Soils
Short- and long-term persistence of pathogenic (i.e., tumor forming) agrobacteria in soil was investigated in six nursery plots with a history of high crown gall incidence. No pathogenic Agrobacterium strains were isolated in soil samples taken in fall and winter in any plots, but such strains were isolated from both bulk soils and weed rhizospheres (over 0.
American Society for Microbiology.
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11. Competition of Octopine-Catabolizing Pseudomonas spp. and Octopine-Type Agrobacterium tumefaciens for Octopine in Chemostats
The ability of two octopine-catabolizing Pseudomonas spp. and two virulent octopine-type Agrobacterium tumefaciens to compete for substrates has been examined in chemostats. In dual cultures with octopine or glutamate as the limiting carbon or nitrogen source, Pseudomonas fluorescens B99A and E175D always dominated over A. tumefaciens B6 or ATCC 15955. The g
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12. Isolation of Different Agrobacterium Biovars from a Natural Oak Savanna and Tallgrass Prairie †
Populations of agrobacteria in excess of 105 CFU/g were recovered from 12 soil and root samples obtained from the Allison Savanna, Minn., a natural oak savanna and tallgrass prairie which has never been disturbed agriculturally. Of 126 strains picked randomly from selective media, 54 were identified as Agrobacterium spp. Biovar 2 strains predominated (35 of