Adenine Sulfate
Mostrando 1-12 de 116 artigos, teses e dissertações.
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1. Assessment of Genetic Fidelity of in vitro Raised Plants in Swertia chirayita through ISSR, RAPD analysis and Peroxidase Profiling during Organogenesis
ABSTRACT An efficient in vitro regeneration protocol for medicinally important herb Swertia chirayita was developed and the genetic fidelity was assessed using RAPD and ISSR markers. The best shoot regeneration was observed on MS basal supplemented with 1.0 mg/L Benzyl amino purine (BAP) in combination with Indole-3-acetic acid (IAA) (0.5 mg/L) that resulted
Braz. arch. biol. technol.. Publicado em: 28/11/2016
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2. Cytokinin Dehydrogenase activity in primary roots and characterization of primary metabolites from leaves and rootlets of Ricinus Communis
The aim of this study was to determine the activity of cytokinin dehydrogenase (CKX) and to measure other biochemical components in the primary leaves and radicles of castor seedlings (BRS Energia) in the initial phase of growth. The crude protein extract obtained after a 1-h extraction from the root tissues of seedlings showed no detectable CKX enzymatic ac
Braz. arch. biol. technol.. Publicado em: 08/11/2013
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3. "Metabólitos secundários biologicamente ativos isolados de esponjas marinhas e do fungo Beauveria felina de origem marinha" / "Biologically active secondary metabolites from Marine Sponges and from the Marine-Derived fungus Beauveria felina"
In this work we report the chemical investigation of bioactive crude extracts obtained from four sponges and two fungal strains of marine origin. The crude extracts of three sponges species (Petromica ciocalyptoides, Topsentia ophiraphidites and Callyspongia sp.) displayed inhibitory activity towards the enzyme adenine fosforribosyl transferase of Leishmania
Publicado em: 2007
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4. MULTIPLICAÇÃO IN VITRO DE MAMOEIRO TAINUNG 01
Experiments were accomplished in establishment and multiplication medium with the objective of evaluating the micropropagation of the papaya tree (Carica papaya L.) Hybrid Tainung 01 , generation F1, being used apical sections and apical sections of lateral buds of juvenile plants, cultivated in greenhouse. In the MS establishment medium, being used apical s
Publicado em: 2006
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5. Inhibition of amino acid transport by ammonium ion in Saccharomyces cerevisiae.
The rate of transport of L-amino acids by Saccharomyces cerevisiae epsilon 1278b increased with time in response to nitrogen starvation. This increase could be prevented by the addition of ammonium sulfate or cycloheximide. A slow time-dependent loss of transport activity was observed when ammonium sulfate (or ammonium sulfate plus cycloheximide) was added t
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6. Orthophosphite-Nicotinamide Adenine Dinucleotide Oxidoreductase from Pseudomonas fluorescens
Information was obtained on the general properties and specificity of orthophosphite-nicotinamide adenine dinucleotide oxidoreductase. The enzyme was extracted from Pseudomonas fluorescens 195 grown in medium containing orthophosphite as the sole source of phosphorus. An enzyme preparation suitable for characterization was obtained from crude extracts by use
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7. Reduced Nicotinamide Adenine Dinucleotide Oxidase Activity and H2O2 Formation of Mycoplasma pneumoniae
Cell-free extracts of Mycoplasma pneumoniae showed two distinct reduced nicotinamide adenine dinucleotide (NADH2) oxidase activities in the supernatant fraction. By ammonium sulfate fractionation and polyacrylamide gel electrophoresis, one activity not requiring flavine co-factors was precipitated by 50 to 70% ammonium sulfate concentration and identified wi
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8. Purification and properties of streptococcal nicotinamide adenine dinucleotide glycohydrolase.
Highly purified streptococcal nicotinamide adenine dinucleotide glycohydrolase (NADase) was obtained by utilizing disodium tetrathionate to protect the enzyme by blocking the sulfhydryl groups of streptococcal proteinase. This was followed by two-step ion-exchange chromatography. The pure enzyme, demonstrated as a single band on sodium dodecyl sulfate/polyac
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9. Sulfate-reducing pathway in Escherichia coli involving bound intermediates.
Although a sulfate-reducing pathway in Escherichia coli involving free sulfite and sulfide has been suggested, it is shown that, as in Chlorella, a pathway involving bound intermediates is also present. E. coli extracts contained a sulfotransferase that transferred the sulfonyl group from a nucleosidephosphosulfate to an acceptor to form an organic thiosulfa
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10. Assimilatory sulfate reduction in an Escherichia coli mutant lacking thioredoxin activity.
An investigation of sulfate reduction in B tsnC*7004, a mutant of Escherichia coli lacking thioredoxin, is reported. Although thioredoxin is indispensable for the adenosine 3'-phosphate 5'-phosphosulfate (PAPS) sulfotransferase reaction under the usual conditions of assay in extracts of wild-type cells, the mutant grew as well as the wild type on sulfate, in
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11. Salt Specificity of a Reduced Nicotinamide Adenine Dinucleotide Oxidase Prepared from a Halophilic Bacterium1
Extracts prepared from a halophilic bacterium contained a reduced nicotinamide adenine dinucleotide (NADH2) oxidase active at high solute concentrations. The cation requirement was nonspecific, since KCl, RbCl, and CsCl replaced NaCl with little or no loss of activity, and NH4Cl was only partially effective. Only LiCl failed to replace NaCl. No specific chlo
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12. Purification and properties of the glutathione reductase of Chromatium vinosum.
The Chromatium vinosum glutathione reductase [NAD(P)H: glutathione disulfide oxidoreductase, EC 1.6.4.2] was purified to apparent homogeneity. The enzyme was found to require reduced nicotinamide adenine dinucleotide (NADH) as a reductant and to be specific for oxidized glutathione (GSSG). The polypeptide molecular weight in sodium dodecyl sulfate was found