6 Aminopenicillanic Acid
Mostrando 1-12 de 62 artigos, teses e dissertações.
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1. Inovações na produção de antibióticos β-lactâmicos
A produção industrial de ácido 6-aminopenicilânico (6-APA) inclui etapas de cultivo de Penicillium chrysogenum, extração com solvente orgânico, cristalização, hidrólise enzimática e precipitação. O interesse industrial e científico em reduzir o número de etapas deste processo tem motivado pesquisadores a buscar processos alternativos para obte
Publicado em: 2009
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2. Improving selectivity and productivity of the enzymatic synthesis of ampicillin with immobilized penicillin G acylase
An experimental design was applied to improve the reaction conditions for enzymatic synthesis of ampicillin from phenylglycine methyl ester (PGME) and 6-aminopenicillanic acid (6-APA), catalyzed by penicillin G acylase from E. coli immobilized on an agarose-glyoxyl derivative. The presence and magnitude of interactions between reaction variables were estimat
Brazilian Journal of Chemical Engineering. Publicado em: 2004-12
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3. Intensificated process of hydrolysis of penicillin G and purification of the products in a multi-stage couter-current reactor / Processo intensificado de hidrolise enzimatica de penicilina G e purificação dos produtos em reator multi-estagio e contra-corrente
This work studies the enzymatic hydrolysis of penicillin G (PenG) into 6-aminopenicillanic acid (6-APA) and phenylacetic acid (PAA). In a multi-stage countercurrent reactor and at low pH, the enzymatic reaction takes place in the aqueous phase and the products are separated between the aqueous phase and organic phase (butyl acetate - BuAc). Furthermore, 6-AP
Publicado em: 2004
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4. Separação de ampicilina produzida enzimaticamente por reação entre éster metílico de fenilglicina e ácido 6-aminopenicilânico.
The separation/concentration process of the products obtained from the enzymatic synthesis of ampicillin (AMP) catalyzed by immobilized penicillin G acylase was the focus of this Thesis. Hydrophobic resin adsorption and isoelectric precipitation were the processes herein evaluated. The antibiotic was produced in the solid phase, from 6-aminopenicillanic acid
Publicado em: 2003
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5. A simplified kinetic model for the side reactions occurring during the enzymatic synthesis of ampicillin
This work presents a kinetic study of the side reactions of the ampicillin enzymatic synthesis, from phenylglycine methyl ester and 6-aminopenicillanic acid using penicillin G acylase immobilized on agarose. A Michaelis-Menten model with competitive inhibition was fitted to initial rates of ester and antibiotic hydrolysis, at pH 6.5 and 25ºC. Inherent kinet
Brazilian Journal of Chemical Engineering. Publicado em: 2000-12
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6. Application of the fluorescamine reaction with 6-aminopenicillanic acid to estimation and detection of penicillin acylase activity.
6-Aminopenicillanic acid may be quantitatively estimated by its reaction with fluorescamine in the concentration range of 1 to 10 micrograms/ml. The difference in reactivity between 6-aminopenicillanic acid and benzylpenicillin, which does not react with fluorescamine, can be used to determine penicillin acylase activity and obtain data on enzyme parameters
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7. Spectrophotometric Method for the Estimation of 6-Aminopenicillanic Acid
A simple, rapid, and sensitive method is described whereby 6-aminopenicillanic acid can be spectrophotometrically determined in the presence of penicillins and their degradation products without prior separation. d-(+)-Glucosamine is used as reagent. The effect of such parameters as pH, temperature, and time of heating on the formation of the chromophore is
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8. Hydrolysis of Phenoxymethyl Penicillin into 6-Aminopenicillanic Acid with Spores of Fusaria
Hydrolysis of phenoxymethyl penicillin into 6-aminopenicillanic acid with spores of fusaria was studied.
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9. Cell surface changes in Pseudomonas aeruginosa PAO4069 in response to treatment with 6-aminopenicillanic acid.
Pseudomonas aeruginosa PAO4096 was induced for beta-lactamases with 6-aminopenicillanic acid. Surface changes concomitant with beta-lactamase induction were monitored. The surface hydrophobicity of the culture increased during exposure to 6-aminopenicillanic acid. The increase was associated with a change in the distribution of the O antigen in the lipopolys
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10. In Vitro Enzymatic Synthesis of New Penicillins Containing Keto Acids as Side Chains
Seven different penicillins containing α-ketobutyric, β-ketobutyric, γ-ketovaleric, α-ketohexanoic, δ-ketohexanoic, ε-ketoheptanoic, and α-ketooctanoic acids as side chains have been synthesized in vitro by incubating the enzymes phenylacetyl coenzyme A (CoA) ligase from Pseudomonas putida and acyl-CoA:6-aminopenicillanic acid acyltransferase from Pen
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11. Inhibition of the Bacillus subtilis Membrane-Bound d-Alanine Carboxypeptidase by 6-Aminopenicillanic Acid Covalently Coupled to Sepharose
An insoluble penicillin derivative, 6-aminopenicillanic acid covalently coupled to Sepharose, was used to investigate the location of the d-alanine carboxypeptidase in the Bacillus subtilis membrane. Only 50% of the enzymatic activity in protoplasts and 74% of that in purified membranes was sensitive, although the purified enzyme could be inhibited completel
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12. Acylation of 6-Aminopenicillanic Acid by Penicillium chrysogenum