Deletion Mutant
Mostrando 1-12 de 4565 artigos, teses e dissertações.
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1. Gene Deletion Leads to Improved Valinomycin Production by Streptomyces sp. CBMAI 2042
The genus Streptomyces represents one of the largest producers of molecules with antibiotic activity. The whole genome sequencing of the endophytic microorganism Streptomyces sp. CBMAI 2042 revealed 35 gene clusters encoding for secondary metabolism including 3 non-ribosomal peptide synthetases (NRPS) and 7 NRPS-hybrids. Combining genome mining and cultivati
J. Braz. Chem. Soc.. Publicado em: 2019-03
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2. Mapping and allelic sequencing of a long sterile lemma trait in rice
ABSTRACT Some outer floral organs are unique in gramineous plants, like the sterile lemma and rudimentary glume in rice. However, their development mechanisms are still poorly understood. In this study, we used 4 mutants with long sterile lemma (LSL), named JF11, JF12, JF13 and JNY-7, to be crossed with Aijiaonante (AJNT) and Nipponbare (NIP), respectively.
Bragantia. Publicado em: 15/05/2017
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3. Characteristics of dr1790 disruptant and its functional analysis in Deinococcus radiodurans
Deinococcus radiodurans (DR) is an extremophile that is well known for its resistance to radiation, oxidants and desiccation. The gene dr1790 of D. radiodurans was predicted to encode a yellow-related protein. The primary objective of the present study was to characterize the biological function of the DR1790 protein, which is a member of the ancient yellow/
Braz. J. Microbiol.. Publicado em: 2015-06
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4. Cgl-SLT2 is required for appressorium formation, sporulation and pathogenicity in Colletotrichum gloeosporioide
The mitogen-activated protein (MAP) kinase pathways has been implicated in the pathogenicity of various pathogenic fungi and plays important roles in regulating pathogenicity-related morphogenesis. This work describes the isolation and characterization of MAP kinase gene, Cgl-SLT2, from Colletotrichum gloeosporioides. A DNA sequence, including 1,633 bp of Cg
Braz. J. Microbiol.. Publicado em: 2013-12
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5. Molecular Analysis of Spinal Muscular Atrophy: a genotyping protocol based on TaqMan® real-time PCR
Spinal muscular atrophy (SMA) is an autosomal recessive inherited disorder caused by alterations in the survival motor neuron I (SMN1) gene. SMA patients are classified as type I-IV based on severity of symptoms and age of onset. About 95% of SMA cases are caused by the homozygous absence of SMN1 due to gene deletion or conversion into SMN2. PCR-based method
Genet. Mol. Biol.. Publicado em: 2012
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6. Efeito de ExoU na ativação de NF-κB e na secreção de IL-8 por células humanas infectadas por Pseudomonas aeruginosa / Effect of Exou on the activation of the NF-κB and the secretion of the IL-8 in human cells infected with Pseudomonas
ExoU, a cytotoxin produced by the opportunistic pathogen Pseudomonas aeruginosa that is translocated into host cell cytosol by the type three secretory system, has been associated with severity of acute infections. We have previously described the potent ExoU proinflammatory activity, which accounts for a market recruitment of neutrophils to infected tissues
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 29/07/2011
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7. Expression of fljB: z66 on a linear plasmid of Salmonella enterica serovar typhi is dependent on FliA and FlhDC and regulated by OmpR
Salmonella enterica serovar Typhi z66-positive strains have two different flagellin genes, fliC:d/j and fljB:z66, located on the chromosome and on a linear plasmid, respectively. To investigate the mechanism underlying the expressional regulation of fljB:z66, gene deletion mutants of the regulators FliA, FlhDC, and OmpR were constructed in this study. The ex
Brazilian Journal of Microbiology. Publicado em: 2010-10
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8. Construction and characterization of a bovine herpesvirus 5 mutant with a deletion of the GI, GE and US9 genes
Bovine herpesvirus 5 (BoHV-5) is a important cause of viral encephalitis in cattle in South America. Within the framework of developing a differential vaccine against BoHV-5, a deletion mutant was constructed based on a Brazilian BoHV-5 isolate. The target of the deletions were genes that code proteins implicated in the neurovirulence of BoHV-5, the glycopro
Publicado em: 2010
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9. Construction and characterization of a glycoprotein e deletion mutant of bovine herpesvirus type 1.2 strain isolated in Brazil / Construção e caracterização de uma amostra de BoHV-1.2 isolada no Brasil com uma deleção no gene da glicoproteína E
Este artigo descreve a construção e caracterização de uma amostra de um herpesvírus bovino tipo 1.2a (BoHV-1.2a) que apresenta uma deleção na região genômica que codifica a glicoproteína E (gE). A deleção gênica foi induzida através da co-transfecção de um fragmento de deleção, contendo as regiões 5´e 3´flanqueadoras da gE, com o DNA vir
Publicado em: 2010
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10. Regulation of enteropathogenic Escherichia coli (EPEC) adhesion by genes related to nutrional shortage and stress. / Regulação da adesão de Escherichia coli enteropatogênica (EPEC) por genes de resposta à limitação nutricional e estresse.
Enteropathogenic E. coli (EPEC) is one of the causes of diarrhea in children. Phosphate (Pi) shortage induces transcription of the genes known as the PHO regulon. These genes are controlled by the Pst system, that is also a high-affinity Pi transporter, and represses PHO expression under Pi-replete conditions. PHO is also controlled by the two-component syst
Publicado em: 2009
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11. Identification and characterization of new modulators of division in B. subtilis / Identificação e caracterização de novos moduladores da divisão em Bacillus subtilis
In prokaryotes, the main form of reproduction is binary fission, which allows the mother-cell to give origin the two daughter-cells, with identical genetic material. In Bacillus subtilis, this process is performed by the divisome, a complex formed for approximately sixteen proteins that leads to the constriction of the membrane and the wall, creating the div
Publicado em: 2009
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12. AvaliaÃÃo da mutaÃÃo ccr5A32 do receptor da B â quimiocina como marcador genÃtico-histÃrico na populaÃÃo de Triunfo Pernambuco / AvaliaÃÃo da mutaÃÃo ccr5A32 do receptor da B â quimiocina como marcador genÃtico-histÃrico na populaÃÃo de Triunfo Pernambuco
The CMKBR5 encodes a 7-transmembrane G-protein-coupled chemokine receptor (CCR5) in macrophages and lymphocytes. A 32 base pair deletion in the coding region of the ccr5 gene has been reported in individuals, homozygous for this deletion, who seem to be resistant to HIV-1 infection. This mutation has a single recent origin in Northeastern Europe, where the h
Publicado em: 2008